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Python is an interpreted, general-purpose high-level programming language whose design philosophy emphasizes code readability. Use the python tag for all Python related questions. If you believe your question may be even more specific, you can include a version specific tag such as python-3.x.
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Serial port data plotter in PyQt
Instead of inheriting from ui_main.Ui_GraphPlotter, I would use the PyQt4.uic module to dynamically load your ui file in the __init__ method:
import os
import pyqtgraph
from PyQt4 import QtGui
from …