There's absolutely no need for an intermediate (dynamic) buffer. Everything you do can be done directly in the client buffer (Buf).

I don't see a reason for fread-ing data. fgets works as good, and spares you from calling ftell and fseek.

The way you remove null chars is suboptimal. I'd keep count of null chars encountered, and shift non-null chars by that count, in a single loop. In any case, I'd factor removal code into a separate function (No Raw Loops rule strikes again).

Looking for a newline is better be done with a standard memchr().

That said, the main code condenses to

char * fgets_and_remove_nulls(char * buf, size_t count, FILE * fp)
{
    if(fgets(buf, size, fp) == NULL) return NULL;
    char * end = memchr(buf, '\n', size);
    if (end == NULL) end = buf + size;
    remove_null_chars(buf, end);
    return buf;
}

There's absolutely no need for an intermediate (dynamic) buffer. Everything you do can be done directly in the client buffer (Buf).

I don't see a reason for fread-ing data. fgets works as good, and spares you from calling ftell and fseek.

The way you remove null chars is suboptimal. I'd keep count of null chars encountered, and shift non-null chars by that count, in a single loop. In any case, I'd factor removal code into a separate function (No Raw Loops rule strikes again).

Looking for a newline is better be done with a standard memchr().

That said, the main code condenses to

char * fgets_and_remove_nulls(char * buf, size_t count, FILE * fp)
{
    memset(buf, 0, size);
    if(fgets(buf, size, fp) == NULL) return NULL;
    char * end = memchr(buf, '\n', size);
    if (end == NULL) end = buf + size;
    remove_null_chars(buf, end);
    return buf;
}